Part:BBa_K2491015:Design

F1 - Flnb, dbaf1, dbfa2 - Fluorene First Part

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 350
  Illegal BsaI.rc site found at 1462
  

Design Notes

The catabolic pathway was synthetized as two polycistronic operons with the codon optimized for E.coli expression, eliminating any prefix and suffix restriction sites and any overlapping sequences. In addition, each operon is under the control of its own promoter parts (insert 1 and insert 2) with its own terminator sequence and with RBS sequences inserted between codons.

Source

Synthetic Genes based on Terrabacter sp. DBF63